henrik.wernerus - Publications List

‪Till Mathan‬ - ‪Google Scholar‬

They have vital functions such as managing cellular functions and communicating between the cell and environment through transporting metabolites, ions, other solutes, etc. An analysis of surface proteomics results reveals novel candidates for intracellular/surface moonlighting proteins in bacteria. Mol Biosyst. (2016) 12:1420–31. doi: 10.1039/c5mb00550g This review will introduce proteomics technology and outline approaches for enriching for cell surface proteins. The mammalian sperm cell at fertilization will be used as an exemplar and we argue that enriching the plasma membrane is critical to map proteins on the cell surface in order to further understand early fertilization events Cell-Surface Proteomics of Naive and Primed Human Pluripotent Stem Cells (A) Plasma membrane profiling and mass spectrometry to quantify cell-surface protein expression in hPSC.

Surface proteomics

2014-03-16 · It is well known that cell surface glycans or glycocalyx play important roles in sperm motility, maturation and fertilization. A comprehensive profile of the sperm surface glycans will greatly facilitate both basic research (sperm glycobiology) and clinical studies, such as diagnostics of infertility. As a group of natural glycan binders, lectin is an ideal tool for cell surface glycan 1 dag sedan · Using a quantitative surface proteomics approach combined with epigenomic/transcriptomic analysis, we uncovered an immunomodulatory effect of DNMTis to facilitate tumor cytolysis by MHC 2020-12-07 · Aebersold R, Mann M. Mass spectrometry-based proteomics. Nature. 2003;422(6928):198–207. CAS Article Google Scholar 5.

This review introd … Comprehensive cell surface proteomics defines markers of classical, intermediate and non-classical monocytes | Scientific Reports Monocytes are a critical component of the cellular innate immune Cell-Surface Proteomics of Naive and Primed Human Pluripotent Stem Cells (A) Plasma membrane profiling and mass spectrometry to quantify cell-surface protein expression in hPSC. Naive H9 hPSC were maintained in t2iLGö on Matrigel-coated plates and primed H9 hPSC were maintained in TeSR-E8 on Vitronectin-coated plates.

Proteomic profiling of extracellular vesicles reveals - Cision

Here we report a novel method of surface proteomics analysis of Pneumocystis murina that reliably detected putative surface proteins that are conserved in Pneumocystis jirovecii. Functional Proteomics Laboratory, Centro de Investigaciones Biológicas (CIB‐CSIC), Madrid, Spain. Functional Proteomics Laboratory, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9. 28040 Madrid, Spain Fax: +34915360432===Search for more papers by this author Cell surface proteolysis is a key mechanism for cell-cell communication and cellular signaling that is commonly dysregulated in human disease.

Surface proteomics on nanoparticles, a step to simplify the

TMT, tandem mass tags. Isolation of specific cell types during stem cell differentiation and reprogramming, and also directly from embryos, is a major technical challenge because few cell-surface proteins are known that can distinguish each cell type. We provide a large-scale proteomic resource of cell-surface proteins for the four embryo-derived stem cell lines. Proteomics is an interdisciplinary domain that has benefitted greatly from the genetic information of various genome projects, including the Human Genome Project. It covers the exploration of proteomes from the overall level of protein composition, structure, and activity.

This information can be Proteomics technologies have matured significantly in recent years and proteomics driven research articles in reproductive biology and medicine are increasingly common. The key challenge is to move from lists of identified proteins to informed understanding of biological function. This review introd … Comprehensive cell surface proteomics defines markers of classical, intermediate and non-classical monocytes | Scientific Reports Monocytes are a critical component of the cellular innate immune Cell-Surface Proteomics of Naive and Primed Human Pluripotent Stem Cells (A) Plasma membrane profiling and mass spectrometry to quantify cell-surface protein expression in hPSC. Naive H9 hPSC were maintained in t2iLGö on Matrigel-coated plates and primed H9 hPSC were maintained in TeSR-E8 on Vitronectin-coated plates. TMT, tandem mass tags. Isolation of specific cell types during stem cell differentiation and reprogramming, and also directly from embryos, is a major technical challenge because few cell-surface proteins are known that can distinguish each cell type. We provide a large-scale proteomic resource of cell-surface proteins for the four embryo-derived stem cell lines.
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Naive H9 hPSC were maintained in t2iLGö on Matrigel-coated plates and primed H9 hPSC were maintained in TeSR-E8 on Vitronectin-coated plates. TMT, tandem mass tags. Cell-surface proteomics identifies lineage-specific markers of embryo-derived stem cells. Rugg-Gunn PJ(1), Cox BJ, Lanner F, Sharma P, Ignatchenko V, McDonald AC, Garner J, Gramolini AO, Rossant J, Kislinger T. Typically, Gram-positive wall proteomics has proved especially challenging as a result of the difficulties in solubilizing this rigid surface.

PY - 2008. Y1 - 2008 2019-03-30 Cell-surface proteins represent attractive targets for therapy, due to their easily-accessible localization and their involvement in essential signaling pathways, often dysregulated in cancer. Despite their clinical appeal, cell-surface proteins are often underrepresented in standard proteomic data sets, due to their poor solubility and lower expression levels compared to intracellular proteins. Se hela listan på news-medical.net Proteomics technologies have matured significantly in recent years and proteomics driven research articles in reproductive biology and medicine are increasingly common.
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‪Christofer Karlsson‬ - ‪Google Scholar‬

This information will become relevant for studying immune infertility. Many of the proteins found on the cell surface are intracellular chaperones or enzymes involved in central metabolic pathways, including some that have previously been shown to have a moonlighting function on the cell surface in at least one species, such as Hsp60/GroEL, DnaK, glyceraldehyde 3-phosphate dehydrogenase, enolase, and fructose 1,6-bisphosphate aldolase. Cell surface is the primary site for sensing extracellular stimuli. The knowledge of the transient changes on the surfaceome upon a perturbation is very important as the initial changed proteins could be driving molecules for some phenotype.

Sveriges lantbruksuniversitet - Primo - SLU-biblioteket

Novel Pneumocystis Antigen Discovery Using Fungal Surface Proteomics. Mingquan Zheng, Yang Cai, Taylor Eddens, David M. Ricks, Jay K. Kolls. G. S. Deepe Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials. Ming-Hui Yang,1,2,3 Shyng-Shiou In order to identify novel AML antigens to be targeted using immunotherapeutic approaches, we have analysed the surface proteome of a collection of 100 Combined gene expression and cell surface proteomics identifies potential therapeutic targets for subtypes of advanced prostate cancer. John K. Lee1 Jul 22, 2020 Opportunities for Low-Input Proteomics in Cancer Biology surface proteins presents itself upon querying the cell proteome for drug targets. Proteomics-based discovery of a novel, structurally unique, and developmentally regulated plasminogen receptor, Plg-RKT, a major regulator of cell surface Jan 10, 2020 Linking proteomics to DNA/RNA sequencing on a single-cell basis (e.g., CITE- seq) is still challenging.

This varies with time and distinct requirements, or stresses, that a cell or organism undergoes Cell surface proteins are major targets of biomedical research due to their utility as cellular markers and their extracellular accessibility for pharmacological intervention. However, information about the cell surface protein repertoire (the surfaceome) of individual cells is only sparsely available.